pX330-EN1680_Sororin-Cterm | Addgene 156451 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pX330-EN1680_Sororin-Cterm

catalog :

156451

citations: 1

Reference
Nora E, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, et al. Molecular basis of CTCF binding polarity in genome folding. Nat Commun. 2020;11:5612 pubmed publisher

product information

Catalog Number :

156451

Product Name :

pX330-EN1680_Sororin-Cterm

article :

doi	10.1038/s41467-020-19283-x
id	28211600
pubmed_id	33154377

bacterial resistance :

Ampicillin

cloning :

backbone

pX330-EN1680_Sororin-Cterm

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth notes :

Please note: Plasmid contains an A to C single nucleotide mutation in the U6 promoter just upstream of the Sororin gRNA sequence, which did not affect sgRNA activity measurably

growth strain :

For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

origin :

pi :

alt_names

CDC5A

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	2610036L13Rik, AL024086, AW536684, C85404
gene	Cdca5
id	67849

genbank_ids

mutation

none

name

Cas9, SORORIN

shRNA_sequence

size

species

10090

Mus musculus