pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP | Addgene 156448 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

catalog :

156448

citations: 2

Reference
Ku H, Park G, Goo J, Lee J, Park T, Shim H, et al. Effects of Transcription-Dependent Physical Perturbations on the Chromosome Dynamics in Living Cells. Front Cell Dev Biol. 2022;10:822026 pubmed publisher
Nora E, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, et al. Molecular basis of CTCF binding polarity in genome folding. Nat Commun. 2020;11:5612 pubmed publisher

product information

Catalog Number :

156448

Product Name :

pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

article :

doi	10.1038/s41467-020-19283-x
id	28211600
pubmed_id	33154377

bacterial resistance :

Ampicillin

cloning :

backbone

pKS070-pCAGGS-3XFLAG-(human)CTCF-eGFP

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid has been found to have dimerized. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you still need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

growth strain :

For transient expression of human CTCF tagged with eGFP

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFAP108, FAP108, MRD21
gene	CTCF
id	10664

genbank_ids

mutation

none

name

human CTCF

shRNA_sequence

size

species

9606

Homo sapiens