pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

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product summary

company name :

Addgene

product type :

cDNA

product name :

pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

catalog :

156433

citations: 1

Reference
Nora E, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, et al. Molecular basis of CTCF binding polarity in genome folding. Nat Commun. 2020;11:5612 pubmed publisher

product information

Catalog Number :

156433

Product Name :

pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

article :

doi	10.1038/s41467-020-19283-x
id	28211600
pubmed_id	33154377

bacterial resistance :

Ampicillin

cloning :

backbone

pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct.

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Mouse Targeting

growth strain :

Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG

origin :

pi :

alt_names

CDC5A

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	2610036L13Rik, AL024086, AW536684, C85404
gene	Cdca5
id	67849

genbank_ids

mutation

none

name

SORORIN, AID

shRNA_sequence

size

species

10090

Mus musculus