This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
catalog :
156415
citations: 1
Reference
Fish J, Flores Suarez C, Boudreau E, Herman A, Gutierrez M, Gustafson D, et al. Somatic Gain of KRAS Function in the Endothelium Is Sufficient to Cause Vascular Malformations That Require MEK but Not PI3K Signaling. Circ Res. 2020;127:727-743 pubmed publisher
product information
Catalog Number :
156415
Product Name :
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
article :
doi10.1161/CIRCRESAHA.119.316500
id28211645
pubmed_id32552404
bacterial resistance :
Ampicillin
cloning :
backbonepDEST_Tol2
backbone_mutation
backbone_origin
backbone_size12248
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Transgenesis
growth strain :
kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
inserts :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterkdrl
sequencing_primer_3KRAS REV2: ctgtattgtcggatctccctc; KRAS FWD2: 5'-gagggagatccgacaatacag;
sequencing_primer_5V5 REV: 5'-CGTAGAATCGAGACCGAGG; V5 FWD: 5'-GGTAAGCCTATCCCTAACCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
NM_033360.4
mutationmutant KRAS4A-G12D
namehuman mutant KRAS4A-G12D
shRNA_sequence
size1417
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmTagBFP2
resistance markers :
5013
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA