This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
catalog :
156413
citations: 1
Reference
Fish J, Flores Suarez C, Boudreau E, Herman A, Gutierrez M, Gustafson D, et al. Somatic Gain of KRAS Function in the Endothelium Is Sufficient to Cause Vascular Malformations That Require MEK but Not PI3K Signaling. Circ Res. 2020;127:727-743 pubmed publisher
product information
Catalog Number :
156413
Product Name :
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
article :
doi10.1161/CIRCRESAHA.119.316500
id28211645
pubmed_id32552404
bacterial resistance :
Ampicillin
cloning :
backbonepDEST_Tol2
backbone_mutation
backbone_origin
backbone_size12211
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Transgenesis
growth strain :
kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites
inserts :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterkdrl
sequencing_primer_3KRAS REV2: ctgtattgtcggatctccctc; KRAS FWD2: 5'-gagggagatccgacaatacag;
sequencing_primer_5V5 REV: 5'-CGTAGAATCGAGACCGAGG; V5 FWD: 5'-GGTAAGCCTATCCCTAACCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
NM_004985.5
mutation
namehuman wild type KRAS4B-WT
shRNA_sequence
size1308
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEYFP
resistance markers :
5013
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA