This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)
catalog :
156406
citations: 1
Reference
Fish J, Flores Suarez C, Boudreau E, Herman A, Gutierrez M, Gustafson D, et al. Somatic Gain of KRAS Function in the Endothelium Is Sufficient to Cause Vascular Malformations That Require MEK but Not PI3K Signaling. Circ Res. 2020;127:727-743 pubmed publisher
product information
Catalog Number :
156406
Product Name :
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)
article :
doi10.1161/CIRCRESAHA.119.316500
id28211645
pubmed_id32552404
bacterial resistance :
Kanamycin
cloning :
backbonepDONR221
backbone_mutation
backbone_origin
backbone_size2545
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Cloning
growth strain :
Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon
inserts :
Stbl3
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI, AscI
cloning_site_5BamHI
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
NM_033360.4
mutationmutant KRAS4A-G12V
namehuman mutant KRAS4A-G12V
shRNA_sequence
size1399
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmScarlet-I
resistance markers :
5013
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA