This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-PGK-flag-RAP1B-V12-neo
catalog :
156175
citations: 1
Reference
Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener R, et al. Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion. Nat Commun. 2021;12:6019 pubmed publisher
product information
Catalog Number :
156175
Product Name :
pLenti-PGK-flag-RAP1B-V12-neo
article :
doi10.1038/s41467-021-26296-7
id28220113
pubmed_id34650052
bacterial resistance :
Ampicillin
cloning :
backbonepLenti PGK Neo DEST (w531-1)
backbone_mutation
backbone_originCampeau lab (addgene #19067)
backbone_size9778
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
flag-RAP1B-V12 cDNA was amplified by PCR and cloned into pENTR DTOPO and transferred by Gateway cloning into Desination vector. bioRxiv 10.1101/792077
growth strain :
Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterhuman PGK
sequencing_primer_3WPRE_R (CATAGCGTAAAAGGAGCAACA)
sequencing_primer_5PGK_F (GTGTTCCGCATTCTGCAAG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneRAP1B
id327708
genbank_ids
NM_175824
mutationchanged Glycine 12 to Valine
nameRAP1B
shRNA_sequence
size609
species
9913
Bos taurus
tags
locationN terminal on insert
tag2xFLAG
plasmid copy :
flag-RAP1B-V12 was cloned using Addgene ID #118323, Flag-Rap1b V12
resistance markers :
2483
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA