Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys | Addgene 15602 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

catalog :

15602

citations: 1

Reference
Tessier P, Lindquist S. Prion recognition elements govern nucleation, strain specificity and species barriers. Nature. 2007;447:556-61 pubmed

product information

Catalog Number :

15602

Product Name :

Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

article :

doi	10.1038/nature05848
id	17
pubmed_id	17495929

bacterial resistance :

Ampicillin

cloning :

backbone

pAED4

backbone_mutation

backbone_origin

backbone_size

3200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35. Lindquist plasmid # 8635.

origin :

pi :

alt_names

Sup35

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36
gene	SUP35
id	851752

genbank_ids

mutation

4 G/A. Four glycines mutated to alanines. The position of the residues in the chimera are 64, 65, 74, 75. The position of the residues in CaNM are 70, 71, 80, 81.

name

Sup35 NM

shRNA_sequence

size

830

species

4932

Saccharomyces cerevisiae

Candida albicans