This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Sup35 Sc/Ca NM Chimera S17R His7 Cys
catalog :
15601
citations: 2
Reference
Saade M, Ferrero D, Blanco Ameijeiras J, Gonzalez Gobartt E, Flores Méndez M, Ruiz Arroyo V, et al. Multimerization of Zika Virus-NS5 Causes Ciliopathy and Forces Premature Neurogenesis. Cell Stem Cell. 2020;27:920-936.e8 pubmed publisher
Tessier P, Lindquist S. Prion recognition elements govern nucleation, strain specificity and species barriers. Nature. 2007;447:556-61 pubmed
product information
Catalog Number :
15601
Product Name :
Sup35 Sc/Ca NM Chimera S17R His7 Cys
article :
doi10.1038/nature05848
id17
pubmed_id17495929
bacterial resistance :
Ampicillin
cloning :
backbonepAED4
backbone_mutation
backbone_origin
backbone_size3200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35. Lindquist plasmid # 8634.
origin :
37
pi :
alt_names
Sup35
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36
geneSUP35
id851752
genbank_ids
mutationS17R. Serine 17 mutated to arginine.
nameSup35 NM
shRNA_sequence
size830
species
4932
Saccharomyces cerevisiae
Candida albicans
tags
locationC terminal on insert
tag7xHis
locationC terminal on insert
tagCysteine
plasmid copy :
Jonathan Weissman's lab.
resistance markers :
293
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA