This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV-F-del Casp9.IRES.GFP
catalog :
15567
citations: 14
Reference
Fujino S, Miyoshi N, Ito A, Hayashi R, Yasui M, Matsuda C, et al. Metastases and treatment-resistant lineages in patient-derived cancer cells of colorectal cancer. Commun Biol. 2023;6:1191 pubmed publisher
Nagae A, Miyoshi N, Fujino S, Horie M, Yachida S, Sasaki M, et al. Cancer Stem Cells Persist Despite Cellular Damage, Emergence of the Refractory Cell Population. Ann Surg Oncol. 2023;: pubmed publisher
Goliwas K, Libring S, Berestesky E, Gholizadeh S, Schwager S, Frost A, et al. Mitochondrial transfer from cancer-associated fibroblasts increases migration in aggressive breast cancer. J Cell Sci. 2023;136: pubmed publisher
Palamarchuk A, Alekseeva N, Streltsova M, Ustiuzhanina M, Kobyzeva P, Kust S, et al. Increased Susceptibility of the CD57- NK Cells Expressing KIR2DL2/3 and NKG2C to iCasp9 Gene Retroviral Transduction and the Relationships with Proliferative Potential, Activation Degree, and Death Induction Response. Int J Mol Sci. 2021;22: pubmed publisher
Schepers A, Jochems F, Lieftink C, Wang L, Pogacar Z, Leite De Oliveira R, et al. Identification of Autophagy-Related Genes as Targets for Senescence Induction Using a Customizable CRISPR-Based Suicide Switch Screen. Mol Cancer Res. 2021;: pubmed publisher
Okamoto T, Duverle D, Yaginuma K, Natsume Y, Yamanaka H, Kusama D, et al. Comparative Analysis of Patient-Matched PDOs Revealed a Reduction in OLFM4-Associated Clusters in Metastatic Lesions in Colorectal Cancer. Stem Cell Reports. 2021;16:954-967 pubmed publisher
Matreyek K, Stephany J, Chiasson M, Hasle N, Fowler D. An improved platform for functional assessment of large protein libraries in mammalian cells. Nucleic Acids Res. 2019;: pubmed publisher
Nguyen T, Nagamune T, Kawahara M. A Suicide Switch Directly Eliminates Intracellular scFv Oligomers in the Cytoplasm of Mammalian Cells. Biotechnol J. 2019;14:e1800350 pubmed publisher
Jacobs C, Badiee R, Lin M. StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins. Nat Methods. 2018;15:523-526 pubmed publisher
Rossignoli F, Grisendi G, Spano C, Golinelli G, Recchia A, Rovesti G, et al. Inducible Caspase9-mediated suicide gene for MSC-based cancer gene therapy. Cancer Gene Ther. 2019;26:11-16 pubmed publisher
Lukianova Hleb E, Yvon E, Shpall E, Lapotko D. All-in-one processing of heterogeneous human cell grafts for gene and cell therapy. Mol Ther Methods Clin Dev. 2016;3:16012 pubmed publisher
Ando M, Nishimura T, Yamazaki S, Yamaguchi T, Kawana Tachikawa A, Hayama T, et al. A Safeguard System for Induced Pluripotent Stem Cell-Derived Rejuvenated T Cell Therapy. Stem Cell Reports. 2015;5:597-608 pubmed publisher
Bugaj L, Choksi A, Mesuda C, Kane R, Schaffer D. Optogenetic protein clustering and signaling activation in mammalian cells. Nat Methods. 2013;10:249-52 pubmed publisher
Straathof K, Pule M, Yotnda P, Dotti G, Vanin E, Brenner M, et al. An inducible caspase 9 safety switch for T-cell therapy. Blood. 2005;105:4247-54 pubmed
product information
Catalog Number :
15567
Product Name :
pMSCV-F-del Casp9.IRES.GFP
article :
doi10.1182/blood-2004-11-4564
id1601
pubmed_id15728125
bacterial resistance :
Ampicillin
cloning :
backboneMSCV
backbone_mutation
backbone_origin
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Based on L. Fan et al (99) Human Gene Therapy
origin :
37
pi :
alt_names
iCaspase9
iCasp9M
FKBP1A
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3cgctttaaatttgcgcatgctagc
sequencing_primer_5cccttgaacctcctcgttcgac
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesFKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE
geneFKBP1A
id2280
genbank_ids
AH002818
NM_001229
mutationFKBP12 F36 to V Truncated Caspase9 (135-416; lacking pro-domain)
nameFKBP12(V36)-p30Caspase9
shRNA_sequence
size1250
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA epitope
locationN terminal on backbone
tagFKBP12
resistance markers :
494
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA