This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKH3-p190
catalog :
15547
citations: 2
Reference
Zhang J, Nguyen A, Miyamoto S, Heller Brown J, McCulloch A, Zhang J. Histamine-induced biphasic activation of RhoA allows for persistent RhoA signaling. PLoS Biol. 2020;18:e3000866 pubmed publisher
Tatsis N, Lannigan D, Macara I. The function of the p190 Rho GTPase-activating protein is controlled by its N-terminal GTP binding domain. J Biol Chem. 1998;273:34631-8 pubmed
product information
Catalog Number :
15547
Product Name :
pKH3-p190
article :
doi10.1074/jbc.273.51.34631
id1581
pubmed_id9852136
bacterial resistance :
Ampicillin
cloning :
backbonepKH3
backbone_mutation
backbone_origin
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Digesting the plasmid with BamHI+EcoRI will excise the entire full-length p190 ORF.
growth strain :
Expression of HA-tagged p190 RhoGAP
origin :
37
pi :
alt_names
p190
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5BamH1
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGrlf1, p190RhoGAP
geneArhgap35
id306400
genbank_ids
M94721
mutation
namep190 GTPase activating protein
shRNA_sequence
size4500
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagTriple HA1 tag
plasmid copy :
The HindIII-EcoRI fragment used to produce the plasmid was isolated from p190 cDNA clone 391 provided by R. Weinberg and J. Settleman (Massachusetts Institute of Technology).
resistance markers :
305
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA