pk7-GR-GFP | Addgene 15534 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pk7-GR-GFP

catalog :

15534

citations: 3

Reference
Lu H, Lei X, Winkler R, John S, Kumar D, Li W, et al. Crosstalk of hepatocyte nuclear factor 4a and glucocorticoid receptor in the regulation of lipid metabolism in mice fed a high-fat-high-sugar diet. Lipids Health Dis. 2022;21:46 pubmed publisher
Fang M, Huang K, Tu W, Chen Y, Pan P, Hsiao W, et al. Chemoproteomic profiling reveals cellular targets of nitro-fatty acids. Redox Biol. 2021;46:102126 pubmed publisher
Carey K, Richards S, Lounsbury K, Macara I. Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import. J Cell Biol. 1996;133:985-96 pubmed

product information

Catalog Number :

15534

Product Name :

pk7-GR-GFP

article :

doi	10.1083/jcb.133.5.985
id	1582
pubmed_id	8655589

bacterial resistance :

Ampicillin

cloning :

backbone

pk7-GFP

backbone_mutation

backbone_origin

backbone_size

5460

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

origin :

pi :

alt_names

pk7-GR-GFP

pk-GR-GFP

GR-GFP

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	XbaI
promoter
sequencing_primer_3
sequencing_primer_5	CAG GTC CAA CTG CAC CTC GGT TC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GCCR, GCR, GCRST, GR, GRL
gene	NR3C1
id	2908

genbank_ids

NM_001018077

mutation

The glucocorticoid receptor was cloned into the XbaI site in pk7-GFP such that the stop codon of GR was deleted and the coding sequence continued in frame with that of the GFP

name

Glucocorticoid receptor

shRNA_sequence

size

2333

species

9606

Homo sapiens