This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTol2CG2-ubi:QFGal4-SV40pA
catalog :
155119
citations: 1
Reference
Burgess J, Burrows J, Sadhak R, Chiang S, Weiss A, D Amata C, et al. An optimized QF-binary expression system for use in zebrafish. Dev Biol. 2020;: pubmed publisher
product information
Catalog Number :
155119
Product Name :
pTol2CG2-ubi:QFGal4-SV40pA
article :
doi10.1016/j.ydbio.2020.07.007
id28211586
pubmed_id32697972
bacterial resistance :
Ampicillin
cloning :
backbonepDestTol2CG2 (Tol2 kit 1.0 #395)
backbone_mutationcmlc2:GFP replaced with cmlc2:membrane-mRFP in reverse orientation
backbone_originEsther Fujimoto, Chien lab
backbone_size6251
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish expression
growth strain :
Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterubiquitin B
sequencing_primer_3GAGGATCATAATCAGCCATA
sequencing_primer_5gctgcaaatagcaggaaacg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesUbi-p63E, im:6892314, si:ch211-202a12.3, ubc, zUBC, zgc:172187
geneubb
id550134
genbank_ids
855828
3875756
mutation
nameubi:QFGal4
shRNA_sequence
size4986
species
7955
Danio rerio
4932
Saccharomyces cerevisiae
32644
Other
Neurospora crassa
tags
resistance markers :
5031
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA