This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
rGSKmyc-pXT7
catalog :
15492
citations: 1
Reference
Dominguez I, Itoh K, Sokol S. Role of glycogen synthase kinase 3 beta as a negative regulator of dorsoventral axis formation in Xenopus embryos. Proc Natl Acad Sci U S A. 1995;92:8498-502 pubmed
product information
Catalog Number :
15492
Product Name :
rGSKmyc-pXT7
article :
doi10.1073/pnas.92.18.8498
id1571
pubmed_id7667318
bacterial resistance :
Ampicillin
cloning :
backbonepXT7-myc
backbone_mutation
backbone_origin
backbone_size3300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Xenopus RNA expression
growth notes :
See author's map for details. 1.4 kb SmaI-HincII rat GSK3beta PCR fragment was cloned from BSSK into SmaI site of pJ3m vector. SalI-BglII digested insert was recloned into XhoI and BglII sites of pXT7; later 3' end was modified to replace SacI, EcoRI, ClaI, and KpnI with XhoI and HindIII.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5XhoI
promoter
sequencing_primer_3SP6 (difficult due to 3' UTR)
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGsk3b
id84027
genbank_ids
mutation
nameGSK-3 beta
shRNA_sequence
size1300
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagmyc
resistance markers :
491
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA