This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2+-Pard3-EGFP-PIF6
catalog :
154914
citations: 1
Reference
Buckley C, Moore R, Reade A, Goldberg A, Weiner O, Clarke J. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell. 2016;36:117-126 pubmed publisher
product information
Catalog Number :
154914
Product Name :
pCS2+-Pard3-EGFP-PIF6
article :
doi10.1016/j.devcel.2015.12.011
id28191641
pubmed_id26766447
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_originRZPD
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
xenopus/avian/zebrafish
growth strain :
Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).
origin :
37
pi :
alt_names
Pard3, Pard3ab
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3M13 Reverse GGAAACAGCTATGACCATG, T3 Forward AATTAACCCTCACTAAAGGG
sequencing_primer_5SP6 Forward ATTTAGGTGACACTATAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesasip, bazooka, par-3, par3, pard3
genepard3ab
id403050
genbank_ids
403050
mutation
namepar-3 family cell polarity regulator alpha, b
shRNA_sequence
size4461
species
7955
Danio rerio
tags
locationC terminal on insert
tagEGFP
locationC terminal on insert
tag1-100 amino acids of Arabidopsis PIF6
resistance markers :
5007
5009
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA