This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2+-Y276H PHYB-CAAX
catalog :
154911
citations: 1
Reference
Buckley C, Moore R, Reade A, Goldberg A, Weiner O, Clarke J. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell. 2016;36:117-126 pubmed publisher
product information
Catalog Number :
154911
Product Name :
pCS2+-Y276H PHYB-CAAX
article :
doi10.1016/j.devcel.2015.12.011
id28191641
pubmed_id26766447
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_originRZPD
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
xenopus/avian/zebrafish
growth strain :
1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.
origin :
37
pi :
alt_names
PHYB
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3M13 Reverse GGAAACAGCTATGACCATG, T3 Forward AATTAACCCTCACTAAAGGG
sequencing_primer_5SP6 Forward ATTTAGGTGACACTATAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
genePHYB
id816394
genbank_ids
816394
mutation1-621 amino acids of Arabidopsis PHYB, Tyrosine residue 276 of PHYB was mutated to Histidine
namePhytochrome B
shRNA_sequence
size2676
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmCherry
locationC terminal on insert
tagCAAX membrane moiety
resistance markers :
5007
5009
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA