This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCW-codon optimized catalytically inactive PHGDH
catalog :
154903
citations: 2
Reference
Van Nyen T, Planque M, van Wagensveld L, Duarte J, Zaal E, Talebi A, et al. Serine metabolism remodeling after platinum-based chemotherapy identifies vulnerabilities in a subgroup of resistant ovarian cancers. Nat Commun. 2022;13:4578 pubmed publisher
Ngo B, Kim E, Osorio Vasquez V, Doll S, Bustraan S, Liang R, et al. Limited Environmental Serine and Glycine Confer Brain Metastasis Sensitivity to PHGDH Inhibition. Cancer Discov. 2020;10:1352-1373 pubmed publisher
product information
Catalog Number :
154903
Product Name :
pCW-codon optimized catalytically inactive PHGDH
article :
doi10.1158/2159-8290.CD-19-1228
id28211558
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCW57-MCS1-P2A-MCS2
backbone_mutation
backbone_origin
backbone_size7943
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses codon optimized catalytically inactive PHGDH in mammalian cells
origin :
37
pi :
alt_names
3-phosphoglycerate dehydrogenase
PHGDH
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterTRE
sequencing_primer_3GAACGGACGTGAAGAATGTG
sequencing_primer_5AGCTCGTTTAGTGAACCGTCAGATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA
genePHGDH
id26227
genbank_ids
NM_006623.4
mutationThree mutations: D175N, R236K, H283A
namePhosphoglycerate dehydrogenase
shRNA_sequence
size1629
species
9606
Homo sapiens
tags
resistance markers :
4993
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA