This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA-HA-PfROM1 (A144G/N145G)
catalog :
154873
citations: 1
Reference
Gandhi S, Baker R, Cho S, Stanchev S, Strisovsky K, Urban S. Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria. Cell Chem Biol. 2020;27:1410-1424.e6 pubmed publisher
product information
Catalog Number :
154873
Product Name :
pcDNA-HA-PfROM1 (A144G/N145G)
article :
doi10.1016/j.chembiol.2020.08.011
id28211545
pubmed_id32888502
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1
origin :
37
pi :
alt_names
Rhomboid protease ROM1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5T7 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPF3D7_0506900
genePF3D7_0506900
id812885
genbank_ids
DQ986368
mutationAlanine 144 changed to Glycine and Asparagine 145 changed to Glycine
namePfROM1
shRNA_sequence
size933
species
100
Synthetic
tags
locationN terminal on insert
tagtriple HA
resistance markers :
4994
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA