This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRG710_lexO-HO_LexA-TF_HIS3MX
catalog :
154819
citations: 1
Reference
Gnügge R, Symington L. Efficient DNA double-strand break formation at single or multiple defined sites in the Saccharomyces cerevisiae genome. Nucleic Acids Res. 2020;48:e115 pubmed publisher
product information
Catalog Number :
154819
Product Name :
pRG710_lexO-HO_LexA-TF_HIS3MX
article :
doi10.1093/nar/gkaa833
id28211532
pubmed_id33053188
bacterial resistance :
Ampicillin
cloning :
backbonepRG203MX
backbone_mutation
backbone_originAddgene plasmid # 64521
backbone_size3833
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
growth strain :
Inducible expression of HO endonuclease in S. cerevisiae
origin :
37
pi :
alt_names
YDL227C
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI (non-unique)
cloning_site_5SacI
promoterlexO_4-CYC1_core
sequencing_primer_3
sequencing_primer_5T7 TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDL227C
geneHO
id851371
genbank_ids
mutation
nameHO endonuclease
shRNA_sequence
size2489
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagFLAG
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI (non-unique)
cloning_site_5XbaI (non-unique)
promoterACT1
sequencing_primer_3M13 Reverse CAGGAAACAGCTATGAC
sequencing_primer_5ACT1 Promoter Forward TATTTCTCTGTCACCCGGCC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameLexA-ER-B112
shRNA_sequence
size2926
species
100
Synthetic
tags
plasmid copy :
LexA TF from FRP880 (Addgene plasmid # 58437).
resistance markers :
5022
tags :
High Copy
terms :
HIS3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA