This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
catalog :
154787
citations: 1
Reference
Biswas J, Rahman R, Gupta V, Rosbash M, Singer R. MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear Organization of Transcription by RNA Editing. iScience. 2020;23:101318 pubmed publisher
product information
Catalog Number :
154787
Product Name :
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGF
P
article :
doi10.1016/j.isci.2020.101318
id28211531
pubmed_id32674054
bacterial resistance :
Ampicillin
cloning :
backbonephage-ubc-nls-ha-tdMCP-gfp (Addgene Plasmid #40649)
backbone_mutation
backbone_origin
backbone_size6200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
origin :
37
pi :
alt_names
ADAR
cloning
clone_methodRestriction Enzyme
cloning_site_3SphI
cloning_site_5AgeI
promoterUBC
sequencing_primer_3GFP_R (5'-GCATCACCTTCACCTTCACC-3')
sequencing_primer_5UBC_F (5'-CGCCGATGATTATATAAGGA-3')
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesDmel_CG12598, ADAR, ADAR1, CG12598, Dmel CG12598, EG:BACN35H14.1, adar, adr, cg12598, dADAR, dAdar, dadar, hypnos-2
geneAdar
id31130
genbank_ids
mutationSynonymized serine to prevent autoediting of ADAR, hyperediting mutation E488Q of ADAR
nameAdenosine deaminse
shRNA_sequence
size1281
species
7227
Drosophila melanogaster
100
Synthetic
tags
locationN terminal on insert
tagstdMCP
locationC terminal on insert
tagV5
locationC terminal on insert
tagGFP
resistance markers :
880
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA