This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA
catalog :
154780
citations: 1
Reference
Meineke B, Heimgärtner J, Eirich J, Landreh M, Elsässer S. Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Cell Rep. 2020;31:107811 pubmed publisher
product information
Catalog Number :
154780
Product Name :
pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA
article :
doi10.1016/j.celrep.2020.107811
id28211530
pubmed_id32579937
bacterial resistance :
Ampicillin
cloning :
backbonepAS
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
dual suppression of rat CRFR1 described by Serfling et al., 2018 (PMID: 29177436 )
growth strain :
plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterEF1
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R, CRH-R 1, CRH-R-1, CRH-R1
geneCrhr1
id58959
genbank_ids
mutation95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA and C55A mutation
nameCRFR1
shRNA_sequence
size1245
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagHA
resistance markers :
4860
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA