This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO.1-TSC2
catalog :
15478
citations: 13
Reference
Fidalgo da Silva E, Abu Khatir B, Drouillard C, Hinch I, Davis G, Sameem M, et al. Tuberin levels during cellular differentiation in brain development. Differentiation. 2023;130:43-50 pubmed publisher
He B, Wang Z, Moreau R. Chylomicron production is repressed by RPTOR knockdown, R-α-lipoic acid and 4-phenylbutyric acid in human enterocyte-like Caco-2 cells. J Nutr Biochem. 2022;108:109087 pubmed publisher
Kaur H, Moreau R. Curcumin steers THP-1 cells under LPS and mTORC1 challenges toward phenotypically resting, low cytokine-producing macrophages. J Nutr Biochem. 2021;88:108553 pubmed publisher
He B, Moreau R. R-α-Lipoic Acid and 4-Phenylbutyric Acid Have Distinct Hypolipidemic Mechanisms in Hepatic Cells. Biomedicines. 2020;8: pubmed publisher
Kaur H, He B, Zhang C, Rodriguez E, Hage D, Moreau R. Piperine potentiates curcumin-mediated repression of mTORC1 signaling in human intestinal epithelial cells: implications for the inhibition of protein synthesis and TNF? signaling. J Nutr Biochem. 2018;57:276-286 pubmed publisher
Shi Q, Viswanadhapalli S, Friedrichs W, Velagapudi C, Szyndralewiez C, Bansal S, et al. Nox4 is a Target for Tuberin Deficiency Syndrome. Sci Rep. 2018;8:3781 pubmed publisher
Thiepold A, Lorenz N, Foltyn M, Engel A, Divé I, Urban H, et al. Mammalian target of rapamycin complex 1 activation sensitizes human glioma cells to hypoxia-induced cell death. Brain. 2017;140:2623-2638 pubmed publisher
Elgendy M, Abdel Aziz A, Renne S, Bornaghi V, Procopio G, Colecchia M, et al. Dual modulation of MCL-1 and mTOR determines the response to sunitinib. J Clin Invest. 2017;127:153-168 pubmed publisher
Roberts J, He B, Erickson A, Moreau R. Improvement of mTORC1-driven overproduction of apoB-containing triacylglyceride-rich lipoproteins by short-chain fatty acids, 4-phenylbutyric acid and (R)-α-lipoic acid, in human hepatocellular carcinoma cells. Biochim Biophys Acta. 2016;1861:166-76 pubmed publisher
Yang C, Matsuura K, Huang N, Robeson A, Huang B, Zhang L, et al. Fatty acid synthase inhibition engages a novel caspase-2 regulatory mechanism to induce ovarian cancer cell death. Oncogene. 2015;34:3264-72 pubmed publisher
Kim E, Kim A, Kim S, Kim H, Chang J, Ahn C, et al. Inhibition of mTORC1 induces loss of E-cadherin through AKT/GSK-3? signaling-mediated upregulation of E-cadherin repressor complexes in non-small cell lung cancer cells. Respir Res. 2014;15:26 pubmed publisher
Munkley J, Rajan P, Lafferty N, Dalgliesh C, Jackson R, Robson C, et al. A novel androgen-regulated isoform of the TSC2 tumour suppressor gene increases cell proliferation. Oncotarget. 2014;5:131-9 pubmed
Vander Haar E, Lee S, Bandhakavi S, Griffin T, Kim D. Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Nat Cell Biol. 2007;9:316-23 pubmed
product information
Catalog Number :
15478
Product Name :
pLKO.1-TSC2
article :
doi10.1038/ncb1547
id1567
pubmed_id17277771
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
Target sequence for human TSC2 is 5'-CACTGGCCTTGGACGGTATTG-3'.
origin :
37
pi :
alt_names
TSC2
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLAM, PPP1R160, TSC4
geneTSC2
id7249
genbank_ids
mutation
nameTSC2 shRNA
shRNA_sequence5 -CACTGGCCTTGGACGGTATTG-3
size59
species
9606
Homo sapiens
tags
resistance markers :
252
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA