This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLX301-ZIM3-KRAB-PYL1
catalog :
154761
citations: 1
Reference
Alerasool N, Segal D, Lee H, Taipale M. An efficient KRAB domain for CRISPRi applications in human cells. Nat Methods. 2020;17:1093-1096 pubmed publisher
product information
Catalog Number :
154761
Product Name :
pLX301-ZIM3-KRAB-PYL1
article :
doi10.1038/s41592-020-0966-x
id28211521
pubmed_id33020655
bacterial resistance :
Ampicillin
cloning :
backbonepLX301
backbone_mutationPYL1 inserted to the 3' end of the Gateway cassette
backbone_originDavid Root lab; Addgene plasmid #25895
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses ZIM3 KRAB domain fused to PYL1
growth temp :
Grow at 30 degrees to avoid recombination
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesZNF264, ZNF657
geneZIM3
id114026
genbank_ids
mutationIncludes ZIM3 aa 1-100
nameZIM3
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
3255
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA