This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
catalog :
154473
citations: 5
Reference
Heinson Y, Han J, Entcheva E. OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology. J Mol Cell Cardiol Plus. 2023;6: pubmed publisher
Han J, Heinson Y, Chua C, Liu W, Entcheva E. CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes. Commun Biol. 2023;6:1236 pubmed publisher
Han J, Heinson Y, Chua C, Liu W, Entcheva E. CRISPRi Gene Modulation and All-Optical Electrophysiology in Post-Differentiated Human iPSC-Cardiomyocytes. bioRxiv. 2023;: pubmed publisher
Ramakrishnan A, Chen L, Burby P, Cadigan K. Wnt target enhancer regulation by a CDX/TCF transcription factor collective and a novel DNA motif. Nucleic Acids Res. 2021;: pubmed publisher
Alerasool N, Segal D, Lee H, Taipale M. An efficient KRAB domain for CRISPRi applications in human cells. Nat Methods. 2020;17:1093-1096 pubmed publisher
product information
Catalog Number :
154473
Product Name :
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
article :
doi10.1038/s41592-020-0966-x
id28211521
pubmed_id33020655
bacterial resistance :
Ampicillin
cloning :
backbonepHR-SFFV-dCas9-BFP-KRAB
backbone_mutationReplaced BFP with mCherry and KOX1 KRAB domain with ZIM3 KRAB domain
backbone_originJonathan Weissman lab (Addgene plasmid #46911)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3
growth temp :
Grow at 30 degrees to avoid recombination
origin :
30
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5ATTGATTGACTGCCCACCTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesZNF264, ZNF657
geneZIM3
id114026
genbank_ids
mutationIncludes ZIM3 aa 1-100
nameZIM3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
resistance markers :
3255
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA