This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLX303-ZIM3-KRAB-dCas9
catalog :
154472
citations: 6
Reference
Yang F, Su W, Chung O, Tracy L, Wang L, Ramsden D, et al. Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesis. Nature. 2023;620:218-225 pubmed publisher
Kaul H, Werschler N, Jones R, Siu M, Tewary M, Hagner A, et al. Virtual cells in a virtual microenvironment recapitulate early development-like patterns in human pluripotent stem cell colonies. Stem Cell Reports. 2023;18:377-393 pubmed publisher
Replogle J, Bonnar J, Pogson A, Liem C, Maier N, Ding Y, et al. Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. elife. 2022;11: pubmed publisher
Xu B, Zhang C, Jiang A, Zhang X, Liang F, Wang X, et al. Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation. J Biol Chem. 2022;:102115 pubmed publisher
Ding L, Schmitt L, Brux M, Sürün D, Augsburg M, Lansing F, et al. DNA methylation-independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins. Life Sci Alliance. 2022;5: pubmed publisher
Alerasool N, Segal D, Lee H, Taipale M. An efficient KRAB domain for CRISPRi applications in human cells. Nat Methods. 2020;17:1093-1096 pubmed publisher
product information
Catalog Number :
154472
Product Name :
pLX303-ZIM3-KRAB-dCas9
article :
doi10.1038/s41592-020-0966-x
id28211521
pubmed_id33020655
bacterial resistance :
Ampicillin
cloning :
backbonepLX303
backbone_mutationdCas9 construct after Gateway recombination sites (C-terminal to the insert)
backbone_originDavid Root
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses ZIM3 KRAB domain fused to the N terminus of dCas9
growth temp :
Grow at 30 degrees to avoid recombination
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesZNF264, ZNF657
geneZIM3
id114026
genbank_ids
mutationIncludes ZIM3 aa 1-100
nameZIM3
shRNA_sequence
size
species
100
Synthetic
tags
locationC terminal on backbone
tagHA
resistance markers :
3255
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA