This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223 SARS-CoV2 TEV-ORF3A
catalog :
154416
citations: 1
Reference
Kim D, Knapp J, Kuang D, Chawla A, Cassonnet P, Lee H, et al. A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. G3 (Bethesda). 2020;10:3399-3402 pubmed publisher
product information
Catalog Number :
154416
Product Name :
pDONR223 SARS-CoV2 TEV-ORF3A
article :
doi10.1534/g3.120.401554
id28211117
pubmed_id32763951
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originRual et al, Genome Res. 14:2128-2135, 2004.
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway-compatible Entry vector, with insert of ORF3A CDS with a TEV sequence at the N-term.
growth strain :
Gateway-compatible Entry vector
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGU280_gp03
geneORF3a
id43740569
genbank_ids
mutationMany synonymous changes due to codon optimization
nameORF3A
shRNA_sequence
size849
species
32644
Other
SARS-CoV-2 isolate Wuhan-Hu-1
tags
plasmid copy :
PCR amplified
resistance markers :
4911
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA