This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEF086 MBP-CREB 127-135-His
catalog :
154075
citations: 1
Reference
Gavagan M, Fagnan E, Speltz E, Zalatan J. The Scaffold Protein Axin Promotes Signaling Specificity within the Wnt Pathway by Suppressing Competing Kinase Reactions. Cell Syst. 2020;10:515-525.e5 pubmed publisher
product information
Catalog Number :
154075
Product Name :
pEF086 MBP-CREB 127-135-His
article :
doi10.1016/j.cels.2020.05.002
id28211438
pubmed_id32553184
bacterial resistance :
Ampicillin
cloning :
backbonepMBP-MG
backbone_mutationModified to include an N-terminal TEV-cleavable MBP tag and a C-terminal His6 tag
backbone_originNew England Biolabs
backbone_size6716
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli
origin :
37
pi :
alt_names
Cyclic AMP-responsive element-binding protein 1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTac
sequencing_primer_3M13F Reverse TGTAAAACGACGGCCAGT
sequencing_primer_5MalE Forward GGTCGTCAGACTGTCGATGAAGCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCREB, CREB-1
geneCREB1
id1385
genbank_ids
mutation
nameCREB1
shRNA_sequence
size27
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMBP
locationC terminal on backbone
tagHis6
resistance markers :
4035
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA