pMG051 MBP- -catenin-His, GST-CK1 | Addgene 154072 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMG051 MBP- -catenin-His, GST-CK1

catalog :

154072

citations: 1

Reference
Gavagan M, Fagnan E, Speltz E, Zalatan J. The Scaffold Protein Axin Promotes Signaling Specificity within the Wnt Pathway by Suppressing Competing Kinase Reactions. Cell Syst. 2020;10:515-525.e5 pubmed publisher

product information

Catalog Number :

154072

Product Name :

pMG051 MBP- -catenin-His, GST-CK1

article :

doi	10.1016/j.cels.2020.05.002
id	28211438
pubmed_id	32553184

bacterial resistance :

Ampicillin

cloning :

backbone

pMBP-MG

backbone_mutation

Modified to include an N-terminal TEV-cleavable MBP tag and a C-terminal His6 tag

backbone_origin

New England Biolabs

backbone_size

6716

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

BamHI restriction site between MBP and B-catenin insert, NotI restriction site between B-catenin insert and His6-tag, SalI restriction site between GST and CK1a insert, PstI restriction site after CK1a stop.

growth strain :

Co-expresses MBP- -catenin-His (human -catenin as a fusion protein with MBP and His- tags) and GST_CK1 in E.coli to produce primed MBP- -catenin-His

origin :

pi :

alt_names

-catenin

Beta-catenin

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	Tac
sequencing_primer_3	GST_N Reverse GAGTGGGTTGCACAAG
sequencing_primer_5	MalE Forward GGTCGTCAGACTGTCGATGAAGCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CTNNB, EVR7, MRD19, NEDSDV, armadillo
gene	CTNNB1
id	1499

genbank_ids

AY463360

mutation

name

CTNNB1

shRNA_sequence

size

2343

species

9606

Homo sapiens