This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SNAP-Dcp2
catalog :
153975
citations: 1
Reference
Lee J, Cathey P, Wu H, Parker R, Voeltz G. Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles. Science. 2020;367: pubmed publisher
product information
Catalog Number :
153975
Product Name :
SNAP-Dcp2
article :
doi10.1126/science.aay7108
id28211428
pubmed_id32001628
bacterial resistance :
Kanamycin
cloning :
backbonepACGFP-c1
backbone_mutationGFP replaced with SNAP tag using NheI and BspEI
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
We amplified GFP-Dcp2 to obtain SNAP-Dcp2
growth strain :
SNAP-tag polypeptide fused to mRNA decapping enzyme 2
origin :
37
pi :
alt_names
Dcp2
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoter
sequencing_primer_3GGTGTGGGAGGTTTTTTAAAGC
sequencing_primer_5GGTGCAGGGCGACCTGGACG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNUDT20
geneDCP2
id167227
genbank_ids
mutation
namemRNA Decapping enzyme 2
shRNA_sequence
size1260
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagSNAP-C1
plasmid copy :
GFP-Dcp2 was a gift from Ross Buchan.
resistance markers :
1740
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA