This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop
catalog :
153956
citations: 1
Reference
Kim D, Knapp J, Kuang D, Chawla A, Cassonnet P, Lee H, et al. A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. G3 (Bethesda). 2020;10:3399-3402 pubmed publisher
product information
Catalog Number :
153956
Product Name :
pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop
article :
doi10.1534/g3.120.401554
id28211117
pubmed_id32763951
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originRual et al, Genome Res. 14:2128-2135, 2004.
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway-compatible Entry vector
growth notes :
Kim et al. 2020 can be accessed at https://doi.org/10.1016/j.cell.2020.04.011
growth strain :
Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGU280_gp08
geneORF7b
id43740574
genbank_ids
mutationMany synonymous changes due to codon optimization
nameORF7B
shRNA_sequence
size60
species
32644
Other
SARS-CoV-2 isolate Wuhan-Hu-1
tags
resistance markers :
4911
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA