This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRepair-SGFP2-CTNNB1
catalog :
153430
citations: 1
Reference
de Man S, Zwanenburg G, van der Wal T, Hink M, van Amerongen R. Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics. elife. 2021;10: pubmed publisher
product information
Catalog Number :
153430
Product Name :
pRepair-SGFP2-CTNNB1
article :
doi10.7554/eLife.66440
id28211397
pubmed_id34190040
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript KS (+)
backbone_mutation
backbone_originstratagene
backbone_size2965
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
Other
HDR donor template
growth notes :
SGFP2 was cloned from pSGFP2-C1 (Dorus Gadella, Plasmid #22881) Homology arms were cloned from genomic HEK293 DNA. Please visit https://www.biorxiv.org/content/10.1101/2020.05.28.120543v1 for bioRxiv preprint.
growth strain :
Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2
origin :
37
pi :
alt_names
CTNNB1
cloning
entrez_gene
aliasesCTNNB, EVR7, MRD19, NEDSDV, armadillo
geneCTNNB1
id1499
genbank_ids
mutation
nameCTNNB1 homology arms and mTurquoise2 coding sequence
shRNA_sequence
size2317
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSGFP2
resistance markers :
4554
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA