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product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-syn-synaptoPAC-minWPRE
catalog :
153085
citations: 1
Reference
Oldani S, Moreno Velasquez L, Faiss L, Stumpf A, Rosenmund C, Schmitz D, et al. SynaptoPAC, an optogenetic tool for induction of presynaptic plasticity. J Neurochem. 2021;156:324-336 pubmed publisher
product information
Catalog Number :
153085
Product Name :
pAAV-syn-synaptoPAC-minWPRE
article :
doi10.1111/jnc.15210
id28211354
pubmed_id33037623
bacterial resistance :
Ampicillin
cloning :
backbonepAAV CW3SL-eGFP (Addgene 61463)
backbone_mutationCamKII promoter was replaced by synapsin promoter. EGFP removed.
backbone_origin
backbone_size3860
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth strain :
Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons
growth temp :
Researchers should avoid repetitive freeze-thaw cycles when handling the DNA, as we found that this can lead to DNA degradation. Recommended growth strains are XL10gold.
origin :
37
pi :
alt_names
photoactivated adenylyl cyclase from Beggiatoa sp.
cloning
entrez_gene
genbank_ids
GU461307
mutation
namebPAC(WT)
shRNA_sequence
size1055
species
32644
Other
Beggiatoa sp.
tags
locationC terminal on insert
tagmyc tag
locationC terminal on insert
tagHis tag
alt_names
syp1
cloning
entrez_gene
aliasesSyp1
geneSyp
id24804
genbank_ids
24804
mutation
nameSynaptophysin
shRNA_sequence
size
species
10116
Rattus norvegicus
32644
Other
tags
locationC terminal on insert
tagmScarlet
resistance markers :
4190
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA