This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pActPL-Gal4DBD
catalog :
15304
citations: 4
Reference
Chio U, Liu Y, Chung S, Shim W, Chandrasekar S, Weiss S, et al. Subunit cooperation in the Get1/2 receptor promotes tail-anchored membrane protein insertion. J Cell Biol. 2021;220: pubmed publisher
Bashir S, Dang T, Rossius J, Wolf J, Kühn R. Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination. BMC Biotechnol. 2020;20:57 pubmed publisher
Kempton H, Goudy L, Love K, Qi L. Multiple Input Sensing and Signal Integration Using a Split Cas12a System. Mol Cell. 2020;78:184-191.e3 pubmed publisher
Luan H, Peabody N, Vinson C, White B. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Neuron. 2006;52:425-36 pubmed
product information
Catalog Number :
15304
Product Name :
pActPL-Gal4DBD
article :
doi10.1016/j.neuron.2006.08.028
id1537
pubmed_id17088209
bacterial resistance :
Ampicillin
cloning :
backbonepActPL
backbone_mutation
backbone_origin
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The RR12EE345L (Zip-) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
origin :
37
pi :
alt_names
RR12EE345L-Gal4DBD
GAL4
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoter
sequencing_primer_3
sequencing_primer_5Gal4-Nterm
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL248C, GAL81
geneGAL4
id855828
genbank_ids
mutation
nameZIP- Gal4DBD
shRNA_sequence
size640
species
tags
resistance markers :
578
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA