This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSH1/S-Fvls-p30Casp9-E
catalog :
15272
citations: 3
Reference
Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, et al. Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells. Sci Rep. 2019;9:3630 pubmed publisher
Bedel A, Béliveau F, Lamrissi Garcia I, Rousseau B, Moranvillier I, Rucheton B, et al. Preventing Pluripotent Cell Teratoma in Regenerative Medicine Applied to Hematology Disorders. Stem Cells Transl Med. 2017;6:382-393 pubmed publisher
Fan L, Freeman K, Khan T, Pham E, Spencer D. Improved artificial death switches based on caspases and FADD. Hum Gene Ther. 1999;10:2273-85 pubmed
product information
Catalog Number :
15272
Product Name :
pSH1/S-Fvls-p30Casp9-E
article :
doi10.1089/10430349950016924
id1530
pubmed_id10515447
bacterial resistance :
Ampicillin
cloning :
backbonepSH1
backbone_mutation
backbone_originGR Crabtree lab
backbone_size3500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
iCaspase9
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NotI, SacII
promoter
sequencing_primer_3cactgcattctagttgtggtttg
sequencing_primer_5gaggtgttacttctgctctaaaagc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
geneCASP9
id842
genbank_ids
AH002818
NM_001229
mutationFKBP12 F36 to VTruncated Caspase9 (135-416; lacking pro-domain)
nameFKBP12(V36)-p30Caspase9
shRNA_sequence
size1250
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA epitope
locationN terminal on insert
tagMyristoylation-targeting domain c-Src (14aa)
resistance markers :
494
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA