This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe-Puro-BRAF-V600E
catalog :
15269
citations: 21
Reference
Chen J, Hug C, Reyes J, Tian C, Gerosa L, Fr xf6 hlich F, et al. Multi-range ERK responses shape the proliferative trajectory of single cells following oncogene induction. Cell Rep. 2023;42:112252 pubmed publisher
Prieto Garcia C, Hartmann O, Reissland M, Braun F, Bozkurt S, Pahor N, et al. USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Mol Oncol. 2022;: pubmed publisher
Schaufler D, Ast D, Tumbrink H, Abedpour N, Maas L, Schwäbe A, et al. Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer. NPJ Precis Oncol. 2021;5:102 pubmed publisher
Buj R, Leon K, Anguelov M, Aird K. Suppression of p16 alleviates the senescence-associated secretory phenotype. Aging (Albany NY). 2021;13:3290-3312 pubmed publisher
Tago K, Funakoshi Tago M, Ohta S, Kawata H, Saitoh H, Horie H, et al. Oncogenic Ras mutant causes the hyperactivation of NF-κB via acceleration of its transcriptional activation. Mol Oncol. 2019;13:2493-2510 pubmed publisher
Wang X, Xu X, Peng C, Qin Y, Gao T, Jing J, et al. BRAFV600E-induced KRT19 expression in thyroid cancer promotes lymph node metastasis via EMT. Oncol Lett. 2019;18:927-935 pubmed publisher
Robb R, Yang L, Shen C, Wolfe A, Webb A, Zhang X, et al. Inhibiting BRAF Oncogene-Mediated Radioresistance Effectively Radiosensitizes BRAFV600E-Mutant Thyroid Cancer Cells by Constraining DNA Double-Strand Break Repair. Clin Cancer Res. 2019;: pubmed publisher
Ng M, Gan Y, Hagen T. Characterisation of cellular effects of Burkholderia pseudomallei cycle inhibiting factor (Cif). Biol Open. 2018;7: pubmed publisher
Liu R, Zhang T, Zhu G, Xing M. Regulation of mutant TERT by BRAF V600E/MAP kinase pathway through FOS/GABP in human cancer. Nat Commun. 2018;9:579 pubmed publisher
Xu Y, Wang X, Chen Y, Chen S, Yang X, Sun Y, et al. Integration of Receptor Tyrosine Kinases Determines Sensitivity to PI3K?-selective Inhibitors in Breast Cancer. Theranostics. 2017;7:974-986 pubmed publisher
Welsch M, Kaplan A, Chambers J, Stokes M, Bos P, Zask A, et al. Multivalent Small-Molecule Pan-RAS Inhibitors. Cell. 2017;168:878-889.e29 pubmed publisher
Ng M, Wang M, Casey P, Gan Y, Hagen T. Activation of MAPK/ERK signaling by Burkholderia pseudomallei cycle inhibiting factor (Cif). PLoS ONE. 2017;12:e0171464 pubmed publisher
Kawakami H, Huang S, Pal K, Dutta S, Mukhopadhyay D, Sinicrope F. Mutant BRAF Upregulates MCL-1 to Confer Apoptosis Resistance that Is Reversed by MCL-1 Antagonism and Cobimetinib in Colorectal Cancer. Mol Cancer Ther. 2016;15:3015-3027 pubmed
Gupta R, Forloni M, Bisserier M, Dogra S, Yang Q, Wajapeyee N. Interferon alpha-inducible protein 6 regulates NRASQ61K-induced melanomagenesis and growth. elife. 2016;5: pubmed publisher
Tachibana N, Cantrup R, Dixit R, Touahri Y, Kaushik G, Zinyk D, et al. Pten Regulates Retinal Amacrine Cell Number by Modulating Akt, Tgf?, and Erk Signaling. J Neurosci. 2016;36:9454-71 pubmed publisher
Hafner M, Niepel M, Chung M, Sorger P. Growth rate inhibition metrics correct for confounders in measuring sensitivity to cancer drugs. Nat Methods. 2016;13:521-7 pubmed publisher
Lakiotaki E, Levidou G, Angelopoulou M, Adamopoulos C, Pangalis G, Rassidakis G, et al. Potential role of AKT/mTOR signalling proteins in hairy cell leukaemia: association with BRAF/ERK activation and clinical outcome. Sci Rep. 2016;6:21252 pubmed publisher
Hellesøy M, Lorens J. Cellular context-mediated Akt dynamics regulates MAP kinase signaling thresholds during angiogenesis. Mol Biol Cell. 2015;26:2698-711 pubmed publisher
Forloni M, Dogra S, Dong Y, Conte D, Ou J, Zhu L, et al. miR-146a promotes the initiation and progression of melanoma by activating Notch signaling. elife. 2014;3:e01460 pubmed publisher
Raabe E, Lim K, Kim J, Meeker A, Mao X, Nikkhah G, et al. BRAF activation induces transformation and then senescence in human neural stem cells: a pilocytic astrocytoma model. Clin Cancer Res. 2011;17:3590-9 pubmed publisher
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed
product information
Catalog Number :
15269
Product Name :
pBabe-Puro-BRAF-V600E
article :
doi10.1016/j.cell.2007.03.052
id1529
pubmed_id17574021
bacterial resistance :
Ampicillin
cloning :
backbonepBabe-puro
backbone_mutation
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
mutationValine 600 to Glutamate (mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
nameBRAF
shRNA_sequence
size2400
species
9606
Homo sapiens
tags
locationC terminal on insert
tagMyc
locationC terminal on insert
tagHis
resistance markers :
184
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA