This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Paxillin-pEGFP
catalog :
15233
citations: 22
Reference
Schmitt M, Colen J, Sala S, Devany J, Seetharaman S, Caillier A, et al. Machine learning interpretable models of cell mechanics from protein images. Cell. 2024;: pubmed publisher
Matsubara H, Fukunaga H, Saito T, Ikezaki K, Iwaki M. A Programmable DNA Origami Nanospring That Reports Dynamics of Single Integrin Motion, Force Magnitude and Force Orientation in Living Cells. ACS Nano. 2023;17:13185-13194 pubmed publisher
Posor Y, Kampyli C, Bilanges B, Ganguli S, Koch P, Wallroth A, et al. Local synthesis of the phosphatidylinositol-3,4-bisphosphate lipid drives focal adhesion turnover. Dev Cell. 2022;: pubmed publisher
Ostrowska Podhorodecka Z, Ding I, Lee W, Tanic J, Abbasi S, Arora P, et al. Vimentin tunes cell migration on collagen by controlling β1 integrin activation and clustering. J Cell Sci. 2021;: pubmed publisher
Wu S, Chen M, Huang J, Zhang F, Lv Z, Jia Y, et al. ORAI2 Promotes Gastric Cancer Tumorigenicity and Metastasis Through PI3K/Akt Signaling and MAPK-dependent Focal Adhesion Disassembly. Cancer Res. 2020;: pubmed publisher
Talayero V, Vicente Manzanares M. Multiparametric Analysis of Focal Adhesions in Bidimensional Substrates. Methods Mol Biol. 2021;2217:27-37 pubmed publisher
Tan S, Chang A, Anderson S, Miller C, Prahl L, Odde D, et al. Regulation and dynamics of force transmission at individual cell-matrix adhesion bonds. Sci Adv. 2020;6:eaax0317 pubmed publisher
Sengupta S, Rothenberg K, Li H, Hoffman B, Bursac N. Altering integrin engagement regulates membrane localization of Kir2.1 channels. J Cell Sci. 2019;132: pubmed publisher
Haage A, Goodwin K, Whitewood A, Camp D, Bogutz A, Turner C, et al. Talin Autoinhibition Regulates Cell-ECM Adhesion Dynamics and Wound Healing In Vivo. Cell Rep. 2018;25:2401-2416.e5 pubmed publisher
Haun F, Neumann S, Peintner L, Wieland K, Habicht J, Schwan C, et al. Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin. Nat Commun. 2018;9:3524 pubmed publisher
Young L, Higgs H. Focal Adhesions Undergo Longitudinal Splitting into Fixed-Width Units. Curr Biol. 2018;28:2033-2045.e5 pubmed publisher
Kubow K, Shuklis V, Sales D, Horwitz A. Contact guidance persists under myosin inhibition due to the local alignment of adhesions and individual protrusions. Sci Rep. 2017;7:14380 pubmed publisher
Chang C, Bijian K, Qiu D, Su J, Saad A, Dahabieh M, et al. Endosomal sorting and c-Cbl targeting of paxillin to autophagosomes regulate cell-matrix adhesion turnover in human breast cancer cells. Oncotarget. 2017;8:31199-31214 pubmed publisher
Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed publisher
Kerstein P, Patel K, Gomez T. Calpain-Mediated Proteolysis of Talin and FAK Regulates Adhesion Dynamics Necessary for Axon Guidance. J Neurosci. 2017;37:1568-1580 pubmed publisher
Wang X, Zheng R, Suo Z, Liu Y, Zhang Z, Ma Z, et al. Activity-dependent dephosphorylation of paxillin contributed to nociceptive plasticity in spinal cord dorsal horn. Pain. 2016;157:652-65 pubmed publisher
Okeke E, Parker T, Dingsdale H, Concannon M, Awais M, Voronina S, et al. Epithelial-mesenchymal transition, IP3 receptors and ER-PM junctions: translocation of Ca2+ signalling complexes and regulation of migration. Biochem J. 2016;473:757-67 pubmed publisher
Sahasrabudhe A, Ghate K, Mutalik S, Jacob A, Ghose A. Formin 2 regulates the stabilization of filopodial tip adhesions in growth cones and affects neuronal outgrowth and pathfinding in vivo. Development. 2016;143:449-60 pubmed publisher
Johnson H, King S, Asokan S, Rotty J, Bear J, Haugh J. F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling. J Cell Biol. 2015;208:443-55 pubmed publisher
Burdyga A, Conant A, Haynes L, Zhang J, Jalink K, Sutton R, et al. cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: effects of PKA and EPAC. Biochim Biophys Acta. 2013;1833:2664-2672 pubmed publisher
Welf E, Ahmed S, Johnson H, Melvin A, Haugh J. Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism. J Cell Biol. 2012;197:105-14 pubmed publisher
Laukaitis C, Webb D, Donais K, Horwitz A. Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. J Cell Biol. 2001;153:1427-40 pubmed
product information
Catalog Number :
15233
Product Name :
Paxillin-pEGFP
article :
doi10.1083/jcb.153.7.1427
id1456
pubmed_id11425873
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N3
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Kpn
cloning_site_5Bgl II
promoter
sequencing_primer_3
sequencing_primer_5CMV immediate early gene forward primer
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesTGFB1I1
genePXN
id395832
genbank_ids
L30099
mutation
namepaxillin
shRNA_sequence
size1700
species
9031
Gallus gallus
tags
locationC terminal on backbone
tagGFP
resistance markers :
208
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA