This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGAL4-CREB S133A
catalog :
15222
citations: 2
Reference
Park B, Jeon J, Go Y, Ham H, Kim J, Yoo E, et al. PDK4 Deficiency Suppresses Hepatic Glucagon Signaling by Decreasing cAMP Levels. Diabetes. 2018;67:2054-2068 pubmed publisher
Delghandi M, Johannessen M, Moens U. The cAMP signalling pathway activates CREB through PKA, p38 and MSK1 in NIH 3T3 cells. Cell Signal. 2005;17:1343-51 pubmed
product information
Catalog Number :
15222
Product Name :
pGAL4-CREB S133A
article :
doi10.1016/j.cellsig.2005.02.003
id1517
pubmed_id16125054
bacterial resistance :
Ampicillin
cloning :
backbonepM
backbone_mutation
backbone_originClontech
backbone_size3500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Wild-type CREB S133A fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the CREB S133A as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.
origin :
37
pi :
alt_names
CREB
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5XhoI/SalI
promoter
sequencing_primer_3
sequencing_primer_5Gal4-N-term primer
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesCreb
geneCreb1
id81646
genbank_ids
mutationSerine 133 mutated to Alanine
nameCREB S133A
shRNA_sequence
size1000
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGal4
plasmid copy :
CREB kindly provided by Richard A. Maurer
resistance markers :
344
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA