pEGFP hsp70 K71E | Addgene 15216 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP hsp70 K71E

catalog :

15216

citations: 4

Reference
Hwang H, Cho Y, Kim J, Yun K, Yoo J, Lee E, et al. Autophagic Inhibition via Lysosomal Integrity Dysfunction Leads to Antitumor Activity in Glioma Treatment. Cancers (Basel). 2020;12: pubmed publisher
Qiu Y, Ye X, Hanson P, Zhang H, Zong J, Cho B, et al. Hsp70-1: upregulation via selective phosphorylation of heat shock factor 1 during coxsackieviral infection and promotion of viral replication via the AU-rich element. Cell Mol Life Sci. 2016;73:1067-84 pubmed publisher
Zhang Y, Zhang X, Shan P, Hunt C, Pandita T, Lee P. A protective Hsp70-TLR4 pathway in lethal oxidant lung injury. J Immunol. 2013;191:1393-403 pubmed publisher
Zeng X, Bhasin S, Wu X, Lee J, Maffi S, Nichols C, et al. Hsp70 dynamics in vivo: effect of heat shock and protein aggregation. J Cell Sci. 2004;117:4991-5000 pubmed

product information

Catalog Number :

15216

Product Name :

pEGFP hsp70 K71E

article :

doi	10.1242/jcs.01373
id	1511
pubmed_id	15367583

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C3

backbone_mutation

backbone_origin

Clontech

backbone_size

4727

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note that Addgene's sequencing results indicate the presence of an extra Arginine, R469, and mutation G370A when compared to the reference sequence M11717.

origin :

pi :

alt_names

hsp70

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	HindIII
promoter
sequencing_primer_3	SV40pA-R
sequencing_primer_5	EGFP-C primer
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	HEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1
gene	HSPA1A
id	3303

genbank_ids

M11717

mutation

K71E, ATPase-deficient mutant.

name

hsp70 K71E

shRNA_sequence

size

species

9606

Homo sapiens