This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP hsp70
catalog :
15215
citations: 31
Reference
Wei Y, Zhuang Y, Zhang Y, Luo L, Yu B, Zeng J. Role of heat shock protein 70 in silibinin-induced apoptosis in bladder cancer. J Cancer. 2024;15:79-89 pubmed publisher
Bracho Vald xe9 s I, Cervantes Villagrana R, Beltr xe1 n Navarro Y, Olgu xed n Olgu xed n A, Escobar Islas E, Carretero Ortega J, et al. Akt Is Controlled by Bag5 through a Monoubiquitination to Polyubiquitination Switch. Int J Mol Sci. 2023;24: pubmed publisher
Kumar P, Kinger S, Dubey A, Jagtap Y, Choudhary A, Prasad A, et al. Trehalose Promotes Clearance of Proteotoxic Aggregation of Neurodegenerative Disease-Associated Aberrant Proteins. Mol Neurobiol. 2023;: pubmed publisher
Niort K, Dancourt J, Boedec E, Al Amir Dache Z, Lavieu G, Tareste D. Cholesterol and Ceramide Facilitate Membrane Fusion Mediated by the Fusion Peptide of the SARS-CoV-2 Spike Protein. ACS Omega. 2023;8:32729-32739 pubmed publisher
Tognoli M, Dancourt J, Bonsergent E, Palmulli R, de Jong O, van Niel G, et al. Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process. Commun Biol. 2023;6:532 pubmed publisher
Pipalia N, Saad S, Subramanian K, Cross A, Al Motawa A, Garg K, et al. HSP90 inhibitors reduce cholesterol storage in Niemann-Pick type C1 mutant fibroblasts. J Lipid Res. 2021;62:100114 pubmed publisher
Actis C, Muzio G, Autelli R. Autophagy Triggers Tamoxifen Resistance in Human Breast Cancer Cells by Preventing Drug-Induced Lysosomal Damage. Cancers (Basel). 2021;13: pubmed publisher
Bonsergent E, Grisard E, Buchrieser J, Schwartz O, Thery C, Lavieu G. Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells. Nat Commun. 2021;12:1864 pubmed publisher
Thakur S, Swiderski K, Chhen V, James J, Cranna N, Islam A, et al. HSP70 drives myoblast fusion during C2C12 myogenic differentiation. Biol Open. 2020;9: pubmed publisher
Bengoechea R, Findlay A, Bhadra A, Shao H, Stein K, Pittman S, et al. Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy. J Clin Invest. 2020;: pubmed publisher
Min K, Shahriyar S, Kwon T. Arylquin 1, a potent Par-4 secretagogue, induces lysosomal membrane permeabilization-mediated non-apoptotic cell death in cancer cells. Toxicol Res. 2020;36:167-173 pubmed publisher
Hwang H, Cho Y, Kim J, Yun K, Yoo J, Lee E, et al. Autophagic Inhibition via Lysosomal Integrity Dysfunction Leads to Antitumor Activity in Glioma Treatment. Cancers (Basel). 2020;12: pubmed publisher
Mishra R, Amanullah A, Upadhyay A, Dhiman R, Dubey A, Singh S, et al. Ubiquitin ligase LRSAM1 suppresses neurodegenerative diseases linked aberrant proteins induced cell death. Int J Biochem Cell Biol. 2020;120:105697 pubmed publisher
Tripathi A, Thangaraj A, Chivero E, Periyasamy P, Callen S, Burkovetskaya M, et al. Antiretroviral-Mediated Microglial Activation Involves Dysregulated Autophagy and Lysosomal Dysfunction. Cells. 2019;8: pubmed publisher
Lim S, Kim D, Kim S. ERK-dependent phosphorylation of the linker and substrate-binding domain of HSP70 increases folding activity and cell proliferation. Exp Mol Med. 2019;51:112 pubmed publisher
Seo S, Min K, Woo S, Kwon T. Z-FL-COCHO, a cathepsin S inhibitor, enhances oxaliplatin-mediated apoptosis through the induction of endoplasmic reticulum stress. Exp Mol Med. 2018;50:107 pubmed publisher
El Buri A, Adams D, Smith D, Tate R, Mullin M, Pyne S, et al. The sphingosine 1-phosphate receptor 2 is shed in exosomes from breast cancer cells and is N-terminally processed to a short constitutively active form that promotes extracellular signal regulated kinase activation and DNA synthesis in fibroblasts. Oncotarget. 2018;9:29453-29467 pubmed publisher
Seo S, Woo S, Lee H, Kim S, Min K, Kwon T. mTORC1/2 inhibitor and curcumin induce apoptosis through lysosomal membrane permeabilization-mediated autophagy. Oncogene. 2018;37:5205-5220 pubmed publisher
Seo S, Woo S, Min K, Kwon T. Z-FL-COCHO, a cathepsin S inhibitor, enhances oxaliplatin-induced apoptosis through upregulation of Bim expression. Biochem Biophys Res Commun. 2018;498:849-854 pubmed publisher
Haystead T. Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors. Methods Mol Biol. 2018;1709:75-86 pubmed publisher
Amanullah A, Mishra R, Upadhyay A, Reddy P, Das R, Mishra A. Indomethacin elicits proteasomal dysfunctions develops apoptosis through mitochondrial abnormalities. J Cell Physiol. 2018;233:1685-1699 pubmed publisher
Seo B, Min K, Woo S, Choe M, Choi K, Lee Y, et al. Inhibition of Cathepsin S Induces Mitochondrial ROS That Sensitizes TRAIL-Mediated Apoptosis Through p53-Mediated Downregulation of Bcl-2 and c-FLIP. Antioxid Redox Signal. 2017;27:215-233 pubmed publisher
Amanullah A, Upadhyay A, Chhangani D, Joshi V, Mishra R, Yamanaka K, et al. Proteasomal Dysfunction Induced By Diclofenac Engenders Apoptosis Through Mitochondrial Pathway. J Cell Biochem. 2017;118:1014-1027 pubmed publisher
Upadhyay A, Amanullah A, Chhangani D, Joshi V, Mishra R, Mishra A. Ibuprofen Induces Mitochondrial-Mediated Apoptosis Through Proteasomal Dysfunction. Mol Neurobiol. 2016;53:6968-6981 pubmed
Halasi M, Váraljai R, Benevolenskaya E, Gartel A. A Novel Function of Molecular Chaperone HSP70: SUPPRESSION OF ONCOGENIC FOXM1 AFTER PROTEOTOXIC STRESS. J Biol Chem. 2016;291:142-8 pubmed publisher
Garrod T, Grubor Bauk B, Gargett T, Li Y, Miller D, Yu W, et al. DNA vaccines encoding membrane-bound or secreted forms of heat shock protein 70 exhibit improved potency. Eur J Immunol. 2014;44:1992-2002 pubmed publisher
Wen W, Liu W, Shao Y, Chen L. VER-155008, a small molecule inhibitor of HSP70 with potent anti-cancer activity on lung cancer cell lines. Exp Biol Med (Maywood). 2014;239:638-45 pubmed publisher
Zhang Y, Zhang X, Shan P, Hunt C, Pandita T, Lee P. A protective Hsp70-TLR4 pathway in lethal oxidant lung injury. J Immunol. 2013;191:1393-403 pubmed publisher
Chhangani D, Mishra A. Mahogunin ring finger-1 (MGRN1) suppresses chaperone-associated misfolded protein aggregation and toxicity. Sci Rep. 2013;3:1972 pubmed publisher
Mishra A, Maheshwari M, Chhangani D, Fujimori Tonou N, Endo F, Joshi A, et al. E6-AP association promotes SOD1 aggresomes degradation and suppresses toxicity. Neurobiol Aging. 2013;34:1310.e11-23 pubmed publisher
Zeng X, Bhasin S, Wu X, Lee J, Maffi S, Nichols C, et al. Hsp70 dynamics in vivo: effect of heat shock and protein aggregation. J Cell Sci. 2004;117:4991-5000 pubmed
product information
Catalog Number :
15215
Product Name :
pEGFP hsp70
article :
doi10.1242/jcs.01373
id1511
pubmed_id15367583
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C3
backbone_mutation
backbone_originClontech
backbone_size4727
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that Addgene's sequencing results indicate the presence of an extra Arginine, R469, when compared to the reference sequence M11717.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1
geneHSPA1A
id3303
genbank_ids
M11717
mutation
namehsp70
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
175
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA