This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Flag-mPIAS3
catalog :
15207
citations: 5
Reference
Dodat F, Cotnoir White D, Dianati E, Vallet A, Mader S, Levesque D. Complex regulation of orphan nuclear receptor Nur77 (Nr4a1) transcriptional activity by SUMO2 and PIASγ. Biochim Biophys Acta Mol Cell Res. 2021;1868:118908 pubmed publisher
Wada H, Suzuki D, Niikura T. Regulation of ALS-Associated SOD1 Mutant SUMOylation and Aggregation by SENP and PIAS Family Proteins. J Mol Neurosci. 2020;: pubmed publisher
Saotome H, Ito A, Kubo A, Inui M. Generation of a Quantitative Luciferase Reporter for Sox9 SUMOylation. Int J Mol Sci. 2020;21: pubmed publisher
He Z, Zhang J, Huang Z, Du Q, Li N, Zhang Q, et al. Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nat Commun. 2018;9:4870 pubmed publisher
Chung C, Liao J, Liu B, Rao X, Jay P, Berta P, et al. Specific inhibition of Stat3 signal transduction by PIAS3. Science. 1997;278:1803-5 pubmed
product information
Catalog Number :
15207
Product Name :
Flag-mPIAS3
article :
doi10.1126/science.278.5344.1803
id1502
pubmed_id9388184
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-Flag
backbone_mutation
backbone_origin
backbone_size3800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that Addgene's sequencing results identified a V580I difference when compared to GenBank reference sequence AAB88902.2. The depositing laboratory has confirmed that plasmid this is exact construct used in the associated publication.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5SalI
promoter
sequencing_primer_3hGH-pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPias3l
genePias3
id229615
genbank_ids
AAB88902.2
mutationV580I
namePIAS3
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on backbone
tagFlag
resistance markers :
477
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA