This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DAP (DOL-AP) (CC#44)
catalog :
15164
citations: 1
| Reference |
|---|
Fields Berry S, Halliday A, Cepko C. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc Natl Acad Sci U S A. 1992;89:693-7 pubmed
|
product information
Catalog Number :
15164
Product Name :
DAP (DOL-AP) (CC#44)
article :
| doi | 10.1073/pnas.89.2.693 |
| id | 1486 |
| pubmed_id | 1731342 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pDOL | ||||
| backbone_mutation | |||||
| backbone_origin | |||||
| backbone_size | |||||
| promoter | |||||
| sequencing_primer_3 | |||||
| sequencing_primer_5 | |||||
| vector_types |
|
growth notes :
Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter. Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.
origin :
37
pi :
|
plasmid copy :
pDOL was a gift from Dr. Richard Mulligan. PLAP was a gift from Dr. John Flanagan and Dr. Philip Leder.
resistance markers :
| 71 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments