This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RCASBP-AP (A) (CC#37)
catalog :
15160
citations: 1
| Reference |
|---|
Fekete D, Cepko C. Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo. Mol Cell Biol. 1993;13:2604-13 pubmed
|
product information
Catalog Number :
15160
Product Name :
RCASBP-AP (A) (CC#37)
article :
| doi | |
| id | 1485 |
| pubmed_id | 8455633 |
bacterial resistance :
Ampicillin
cloning :
| backbone | RCASBP (A) | |||
| backbone_mutation | ||||
| backbone_origin | ||||
| backbone_size | 11600 | |||
| promoter | ||||
| sequencing_primer_3 | ||||
| sequencing_primer_5 | ||||
| vector_types |
|
growth notes :
In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(A) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').
origin :
37
pi :
|
plasmid copy :
RCASBP(A) provided by S. Hughes.
resistance markers :
| 71 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments