pLIA CRX EnR (CC#145) | Addgene 15155 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLIA CRX EnR (CC#145)

catalog :

15155

citations: 1

Reference
Furukawa T, Morrow E, Cepko C. Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation. Cell. 1997;91:531-41 pubmed

product information

Catalog Number :

15155

Product Name :

pLIA CRX EnR (CC#145)

article :

doi	10.1016/S0092-8674(00)80439-0
id	1472
pubmed_id	9390562

bacterial resistance :

Ampicillin

cloning :

backbone

pLIA

backbone_mutation

backbone_origin

Cepko Lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

For the CRX-EnR fusion construct, a 302 bp XmaI fragment (amino acids 7 108) containing the Crx homeobox was fused to the EnR domain. To make the CRX-EnR fusion, the XmaI Crx fragment, an EcoRI-XmaI linker encoding amino acids 2 6 with a NcoI site at the methionine of position 2, an XmaI-ClaI linker, and a ClaI-NotI (partial digestion) 930 bp fragment of the EnR were ligated into a shuttle vector, pSLAX21. The ClaI site inside this construct was designed so that the entire CRX-EnR fusion could be excised as a 1.3 kb ClaI fragment from pSLAX21/CRX-EnR. The ClaI fragment was ligated into pLIA.

origin :

pi :

alt_names

cone-rod homeobox containing gene

cloning

clone_method	Restriction Enzyme
cloning_site_3	ClaI/SnaBI
cloning_site_5	ClaI/SnaBI
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	Crx1
gene	Crx
id	12951

genbank_ids

mutation

amino acids 7 108, containing the CRX homeobox

name

CRX

shRNA_sequence

size

1300

species

10090

Mus musculus