This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RCAS DNhRARalpha (CC#1865)
catalog :
15153
citations: 2
Reference
Bonney S, Harrison Uy S, Mishra S, MacPherson A, Choe Y, Li D, et al. Diverse Functions of Retinoic Acid in Brain Vascular Development. J Neurosci. 2016;36:7786-801 pubmed publisher
Sen J, Harpavat S, Peters M, Cepko C. Retinoic acid regulates the expression of dorsoventral topographic guidance molecules in the chick retina. Development. 2005;132:5147-59 pubmed
product information
Catalog Number :
15153
Product Name :
RCAS DNhRARalpha (CC#1865)
article :
doi10.1242/dev.02100
id1484
pubmed_id16251210
bacterial resistance :
Ampicillin
cloning :
backboneRCASBP (A)
backbone_mutation
backbone_origin
backbone_size11600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
Other
Avian expression
growth notes :
RARa was PCR-amplified using GCAGAAGACCCCATGGCCAGCAACAGCAGCTCCT as the 5' primer and CCGGAATTCCAACATTTCCTGGATGAGAGGCGG as the 3' primer. The PCR product was digested with BbsI and EcoRI, and subcloned into the NcoI/EcoRI sites of pSlax21 to generate pSlax21-DNhRAR. The ClaI fragment of pSlax21-DNhRAR was then subcloned into the RCASBP(A) vector.
origin :
37
pi :
alt_names
RARa
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5ClaI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNR1B1, RAR, RARalpha
geneRARA
id5914
genbank_ids
mutationDominant negative. Truncated at aa403, so lacks the C-terminal transcriptional activation domain.
nameRAR alpha (dom neg)
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
RAR alpha provided by Dr. Ronald Evans.
resistance markers :
71
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA