This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHes1-DN-GFP (CC#110)
catalog :
15134
citations: 1
Reference
Furukawa T, Mukherjee S, Bao Z, Morrow E, Cepko C. rax, Hes1, and notch1 promote the formation of Müller glia by postnatal retinal progenitor cells. Neuron. 2000;26:383-94 pubmed
product information
Catalog Number :
15134
Product Name :
pHes1-DN-GFP (CC#110)
article :
doi10.1016/S0896-6273(00)81171-X
id1475
pubmed_id10839357
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-MN-IRES-E-GFP
backbone_mutation
backbone_originGary Nolan, Stanford
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Dominant negative Hes1. Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.
growth temp :
Grow in Stbl3 at 30C
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases
geneHes1
id29577
genbank_ids
mutationE43A, K44A, R47A
nameHes1
shRNA_sequence
size1100
species
10116
Rattus norvegicus
tags
resistance markers :
71
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA