pHes1-GFP (CC#109) | Addgene 15133 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHes1-GFP (CC#109)

catalog :

15133

citations: 1

Reference
Furukawa T, Mukherjee S, Bao Z, Morrow E, Cepko C. rax, Hes1, and notch1 promote the formation of Müller glia by postnatal retinal progenitor cells. Neuron. 2000;26:383-94 pubmed

product information

Catalog Number :

15133

Product Name :

pHes1-GFP (CC#109)

article :

doi	10.1016/S0896-6273(00)81171-X
id	1475
pubmed_id	10839357

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-MN-IRES-E-GFP

backbone_mutation

backbone_origin

Gary Nolan, Stanford

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

An EcoRI-AhaIII fragment containing the entire Hes1 coding region was blunted and subcloned in the blunted BamHI site of the pGFP virus vector. Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.

growth temp :

Grow at 30C in Stbl3

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases
gene	Hes1
id	29577

genbank_ids

mutation

name

Hes1

shRNA_sequence

size

1100

species

10116

Rattus norvegicus