This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTBNde(min)
catalog :
15125
citations: 6
Reference
Bonet Fernández J, Aroca Aguilar J, Corton M, Ramírez A, Alexandre Moreno S, García Antón M, et al. CPAMD8 loss-of-function underlies non-dominant congenital glaucoma with variable anterior segment dysgenesis and abnormal extracellular matrix. Hum Genet. 2020;: pubmed publisher
Alió Del Barrio J, Chung D, Al Shymali O, Barrington A, Jatavallabhula K, Swamy V, et al. Punctiform and Polychromatic Pre-Descemet Corneal Dystrophy: Clinical Evaluation and Identification of the Genetic Basis. Am J Ophthalmol. 2020;212:88-97 pubmed publisher
Balestra D, Giorgio D, Bizzotto M, Fazzari M, Ben Zeev B, Pinotti M, et al. Splicing Mutations Impairing CDKL5 Expression and Activity Can be Efficiently Rescued by U1snRNA-Based Therapy. Int J Mol Sci. 2019;20: pubmed publisher
Fujiwara T, Takeda N, Hara H, Morita H, Kishihara J, Inuzuka R, et al. Distinct variants affecting differential splicing of TGFBR1 exon 5 cause either Loeys-Dietz syndrome or multiple self-healing squamous epithelioma. Eur J Hum Genet. 2018;26:1151-1158 pubmed publisher
Hwang I, Cao D, Na Y, Kim D, Zhang T, Yao J, et al. Far Upstream Element-Binding Protein 1 Regulates LSD1 Alternative Splicing to Promote Terminal Differentiation of Neural Progenitors. Stem Cell Reports. 2018;10:1208-1221 pubmed publisher
Pagani F, Stuani C, Tzetis M, Kanavakis E, Efthymiadou A, Doudounakis S, et al. New type of disease causing mutations: the example of the composite exonic regulatory elements of splicing in CFTR exon 12. Hum Mol Genet. 2003;12:1111-20 pubmed
product information
Catalog Number :
15125
Product Name :
pTBNde(min)
article :
doi10.1093/hmg/ddg131
id1471
pubmed_id12719375
bacterial resistance :
Ampicillin
cloning :
backbonepBlueScript II KS
backbone_mutationmodified to include an alpha-globin-fibronectin EDB minigene in which transcription is driven from the minimal -globin promoter and SV40 enhancer
backbone_origin
backbone_size2960
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Hybrid minigene used for splicing assay for pre-mRNA processing. Contains a unique NdeI site for subcloning sequence of interest.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5SacI
promoteralpha-globin
sequencing_primer_3M13 REV
sequencing_primer_5M13 DIR
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF
geneFN1
id2335
genbank_ids
mutation
namealpha-globin EDB fibronectin minigene
shRNA_sequence
size3151
species
9606
Homo sapiens
tags
resistance markers :
375
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA