This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
V45 pHIPPY PGL3 Luciferase
catalog :
15099
citations: 1
Reference
Kaykas A, Moon R. A plasmid-based system for expressing small interfering RNA libraries in mammalian cells. BMC Cell Biol. 2004;5:16 pubmed
product information
Catalog Number :
15099
Product Name :
V45 pHIPPY PGL3 Luciferase
article :
doi10.1186/1471-2121-5-16
id1457
pubmed_id15119963
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepHIPPY
backbone_mutation
backbone_originMoon Lab
backbone_size2167
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
RNAi
growth notes :
Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case. Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoter
sequencing_primer_3
sequencing_primer_5H1 primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutation
namePGL3 luciferase siRNA
shRNA_sequence
size19
species
tags
resistance markers :
348
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA