This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PB-CAG-mRuby3-Gal8-P2A-Zeo
catalog :
150815
citations: 4
Reference
xd6 zt xfc rk x, Lessl A, H xf6 hn M, Wuttke S, Nielsen P, Wagner E, et al. Peptide nucleic acid-zirconium coordination nanoparticles. Sci Rep. 2023;13:14222 pubmed publisher
Borgo G, Burke T, Tran C, Lo N, Engström P, Welch M. A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes. Nat Commun. 2022;13:3656 pubmed publisher
Rui Y, Wilson D, Tzeng S, Yamagata H, Sudhakar D, Conge M, et al. High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA. Sci Adv. 2022;8:eabk2855 pubmed publisher
Millarte V, Schlienger S, Kälin S, Spiess M. Rabaptin5 targets autophagy to damaged endosomes and Salmonella vacuoles via FIP200 and ATG16L1. EMBO Rep. 2022;23:e53429 pubmed publisher
product information
Catalog Number :
150815
Product Name :
PB-CAG-mRuby3-Gal8-P2A-Zeo
article :
doi10.1126/sciadv.abk2855
id28227744
pubmed_id34985952
bacterial resistance :
Ampicillin
cloning :
backbonePB-CAG-GAPd2
backbone_mutationIn-frame insertion of Gal8 to form c-terminus fusion protein with mRuby3 with P2A ribosomal skip site followed by zeocin resistance for mammalian selection
backbone_origin
backbone_size6466
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein
origin :
37
pi :
alt_names
Galectin-8
mRuby3
LGALS8
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5AgeI
promoterCAG
sequencing_primer_3GCAACATATGCCCATATGCTGGC
sequencing_primer_5GCAACGTGCTGGTTATTGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGal-8, PCTA-1, PCTA1, Po66-CBP
geneLGALS8
id3964
genbank_ids
3964
mutation
namemRuby3-Gal8-P2A-Zeo
shRNA_sequence
size2241
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on backbone
tagGal8 is fused to the c-terminus of mRuby3
resistance markers :
4068
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA