This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RIP Cre-ER (DM#265)
catalog :
15029
citations: 3
Reference
Mulder N, Havinga R, Kluiver J, Groen A, Kruit J. AAV8-mediated gene transfer of microRNA-132 improves beta cell function in mice fed a high-fat diet. J Endocrinol. 2019;240:123-132 pubmed publisher
Guay C, Kruit J, Rome S, Menoud V, Mulder N, Jurdzinski A, et al. Lymphocyte-Derived Exosomal MicroRNAs Promote Pancreatic β Cell Death and May Contribute to Type 1 Diabetes Development. Cell Metab. 2019;29:348-361.e6 pubmed publisher
Dor Y, Brown J, Martinez O, Melton D. Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature. 2004;429:41-6 pubmed
product information
Catalog Number :
15029
Product Name :
RIP Cre-ER (DM#265)
article :
doi10.1038/nature02520
id1433
pubmed_id15129273
bacterial resistance :
Ampicillin
cloning :
backbonemodified pBluescript II KS
backbone_mutation
backbone_origin
backbone_size6700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
Other
for making transgenic constructs
growth notes :
The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. The RIP CreER construct was generated by fusing a 0.66-kilobase SmaI HindIII fragment of the RIP2 promoter to a minimal hsp68 promoter, and placing the chimaeric promoter upstream of CreER(TM). See Author's Map for more information.
origin :
37
pi :
alt_names
insulin promoter
ins2
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCP-II
geneIns2
id24506
genbank_ids
mutation
nameRat insulin promoter (RIP)
shRNA_sequence
size700
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagCre-ER (TM)
plasmid copy :
Cre-ER(TM) was a gift from Andy McMahon.
resistance markers :
327
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA